Get up to $10,000 to pay for Transgenic Core services from the CTSC.
Cryopreservation: How, who and how much? A comparison.
The Hanson Lab's PEPCK-Cmus Mouse: BBC News Story
The Warman Lab's Conditional High Bone Mass Lrp5 Mice: New York Times Story

     The Case Transgenic Core creates transgenic mice, knockout mice (null mutations, knockins, targeted point mutations, humanized genes, conditional knockouts) and chimeric mice, provide rederivation, cryopreservation services and establish embryonic stem cell lines for the Cleveland Biomedical Research Community.

     Services are contracted on a fee-for-service, first-come-first-serve basis. Our rates are low, our wait times are short, and we have an outstanding record of success with many publications resulting from our work.

     The Transgenic Facility is supported by user fees, the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center and the Clinical & Translational Science Collaborative (CTSC).

     The Case Transgenic and Targeting Facility office is located in Wolstein Research Bldg 2514.

Personnel:
Weihong JiangStaff216 368-2528wxj13@case.edu
David LePageStaff216 368-2528dfl@case.edu
Rachel MannManager216 368-2528rachel.mann@case.edu
Ron ConlonDirector216 368-1826rac14@case.edu



Transgenic Facility Wait Times:
ServiceTime to Job Start* ( Current as of 1/28/12)
Transgenic Mice3 weeks
Chimeric Mice6 weeks
Rederived Miceimmediately
Cryopreservationimmediately
Gene Targeting1 month
*after receipt and validation of materials and information

 

   Transgenic Core Services: Information:
New: IBC review of the creation of new transgenic and chimeric mice is now integrated into our electronic ordering system. Place an order and the IBC will review your request: there is no need to separately apply for IBC approval if you are placing an order with us.





Use Google to search the CWRU Transgenic Facility site

Transgenic Core Staff (Left to Right): Ron Conlon, Rachel Mann, David LePage, Weihong Jiang

 

Transgenic and Knockout Mice Made by the Transgenic Facility, Published in 2010 and 2011:
  1. Deneris ES. (2011) Molecular genetics of mouse serotonin neurons across the lifespan. Neuroscience 197:17-27

  2. Niziolek PJ, Farmer TL, Cui Y, Turner CH, Warman ML, Robling AG. (2011) High-bone-mass-producing mutations in the Wnt signaling pathway result in distinct skeletal phenotypes. Bone

  3. Hodges CA, Grady BR, Mishra K, Cotton CU, Drumm ML. (2011) Cystic fibrosis growth retardation is not correlated with loss of Cftr in the intestinal epithelium. Am J Physiol Gastrointest Liver Physiol. 301(3):G528-36.

  4. Vogt DL, Thomas D, Galvan V, Bredesen DE, Lamb BT, Pimplikar SW. (2011) Abnormal neuronal networks and seizure susceptibility in mice overexpressing the APP intracellular domain. Neurobiol Aging. 32(9):1725-9

  5. Gutierrez DV, Mark MD, Masseck O, Maejima T, Kuckelsberg D, Hyde RA, Krause M, Kruse W, Herlitze S. (2011) Optogenetic Control of Motor Coordination by Gi/o Protein-coupled Vertebrate Rhodopsin in Cerebellar Purkinje Cells. J Biol Chem. 286(29):25848-58.

  6. Szczepanek K, Chen Q, Derecka M, Salloum FN, Zhang Q, Szelag M, Cichy J, Kukreja RC, Dulak J, Lesnefsky EJ, Larner AC. (2011) Mitochondrial-targeted signal transducer and activator of transcription (STAT3) protects against ischemia-induced changes in the electron transport chain and the generation of reactive oxygen species. J Biol Chem. 2011

  7. Depuy SD, Kanbar R, Coates MB, Stornetta RL, Guyenet PG. (2011) Control of breathing by raphe obscurus serotonergic neurons in mice. J Neurosci. 2011 Feb 9;31(6):1981-90.

  8. Zhu Q, Sun W, Okano K, Chen Y, Zhang N, Maeda T, Palczewski K. (2011) A sponge transgenic mouse model reveals important roles for the miRNA-183/96/182 cluster in post-mitotic photoreceptors of the retina. J Biol Chem.

  9. Kyono A, Avishai N, Ouyang Z, Landreth GE, Murakami S. (2011) FGF and ERK signaling coordinately regulate mineralization-related genes and play essential roles in osteocyte differentiation. J Bone Miner Metab.

  10. Cui Y, Niziolek PJ, Macdonald BT, Zylstra CR, Alenina N, Robinson DR, Zhong Z, Matthes S, Jacobsen CM, Conlon RA, Brommage R, Liu Q, Mseeh F, Powell DR, Yang QM, Zambrowicz B, Gerrits H, Gossen JA, He X, Bader M, Williams BO, Warman ML, Robling AG. (2011) Lrp5 functions in bone to regulate bone mass. Nat Med 17(6):684-91


  11. Berger DS, Moyer M, Kliment GM, van Lunteren E, Ladd AN (2011) Expression of a Dominant Negative CELF Protein In Vivo Leads to Altered Muscle Organization, Fiber Size, and Subtype. PLoS One 6(4):e19274.

  12. Hawthorne AL, Hu H, Kundu B, Steinmetz MP, Wylie CJ, Deneris ES, Silver J. (2011) The Unusual Response of Serotonergic Neurons after CNS Injury: Lack of Axonal Dieback and Enhanced Sprouting within the Inhibitory Environment of the Glial Scar. J Neurosci 31(15):5605-5616.

  13. Song NN, Xiu JB, Huang Y, Chen JY, Zhang L, Gutknecht L, Lesch KP, Li H, Ding YQ. (2011) Adult raphe-specific deletion of Lmx1b leads to central serotonin deficiency. PLoS One. 6(1):e15998.

  14. Kiyasova V, Fernandez SP, Laine J, Stankovski L, Muzerelle A, Doly S, Gaspar P. (2011) A Genetically Defined Morphologically and Functionally Unique Subset of 5-HT Neurons in the Mouse Raphe Nuclei. J Neurosci. 31(8):2756-68.

  15. Liu Y, Jiang Y, Si Y, Kim JY, Chen ZF, Rao Y. (2011) Molecular regulation of sexual preference revealed by genetic studies of 5-HT in the brains of male mice. Nature 472, 95-99

  16. Mark MD, Maejima T, Kuckelsberg D, Yoo JW, Hyde RA, Shah V, Gutierrez D, Moreno RL, Kruse W, Noebels JL, Herlitze S. (2011) Delayed Postnatal Loss of P/Q-Type Calcium Channels Recapitulates the Absence Epilepsy, Dyskinesia, and Ataxia Phenotypes of Genomic Cacna1A Mutations. J Neurosci 31(11):4311-26.

  17. Lu X, Guo H, Molter J, Miao H, Gerber L, Hu Y, Barnes EL, Vogel H, Lee Z, Luo G, Wang B. (2011) Alpha-fetoprotein-thymidine kinase-luciferase knockin mice: A novel model for dual modality longitudinal imaging of tumorigenesis in liver. J Hepatol. 55(1):96-102

  18. Mester JL, Tilot AK, Rybicki LA, Frazier TW 2nd, Eng C. (2011) Analysis of prevalence and degree of macrocephaly in patients with germline PTEN mutations and of brain weight in Pten knock-in murine model. Eur J Hum Genet. 19(7):763-8

  19. Fyffe-Maricich SL, Karlo JC, Landreth GE, Miller RH. (2011) The ERK2 Mitogen-Activated Protein Kinase Regulates the Timing of Oligodendrocyte Differentiation. J Neurosci 31(3):843-50.

  20. Newbern JM, Li X, Shoemaker SE, Zhou J, Zhong J, Wu Y, Bonder D, Hollenback S, Coppola G, Geschwind DH, Landreth GE, Snider WD. (2011) Specific Functions for ERK/MAPK Signaling during PNS Development. Neuron 69(1):91-105

  21. Ghosal K, Pimplikar SW. (2011) Aging and excitotoxic stress exacerbate neural circuit reorganization in amyloid precursor protein intracellular domain transgenic mice. Neurobiol Aging 32(12):2320.e1-9

  22. Gulen MF, Kang Z, Bulek K, Youzhong W, Kim TW, Chen Y, Altuntas CZ, Sass Bak-Jensen K, McGeachy MJ, Do JS, Xiao H, Delgoffe GM, Min B, Powell JD, Tuohy VK, Cua DJ, Li X. (2010) The receptor SIGIRR suppresses Th17 cell proliferation via inhibition of the interleukin-1 receptor pathway and mTOR kinase activation. Immunity. 32(1):54-66.

  23. Wang Y, Martin JF, Bai CB. (2010) Direct and indirect requirements of Shh/Gli signaling in early pituitary development. Dev Biol. 348(2):199-209

  24. Wang YC, Zuraek MB, Kosaka Y, Ota Y, German MS, Deneris ES, Bergsland EK, Donner DB, Warren RS, Nakakura EK. (2010) The ETS oncogene family transcription factor FEV identifies serotonin-producing cells in normal and neoplastic small intestine. Endocr Relat Cancer 17(1):283-91.

  25. Oh E, Maejima T, Liu C, Deneris E, Herlitze S. (2010) Substitution of 5-HT1A receptor signaling by a light-activated G protein-coupled receptor. J Biol Chem 285(40):30825-36.

  26. Hu Y, Lu X, Luo G (2010) Effect of Recql5 deficiency on the intestinal tumor susceptibility of Apc(min) mice. World J Gastroenterol 16(12):1482-6.

  27. Buchanan GF, Richerson GB (2010) Central serotonin neurons are required for arousal to CO2. Proc Natl Acad Sci U S A. 107(37):16354-9

  28. Schnetz MP, Handoko L, Akhtar-Zaidi B, Bartels CF, Pereira CF, Fisher AG, Adams DJ, Flicek P, Crawford GE, Laframboise T, Tesar P, Wei CL, Scacheri PC. (2010 CHD7 targets active gene enhancer elements to modulate ES cell-specific gene expression. PLoS Genet. 6(7):e1001023.

  29. Roy D, Gargesha M, Steyer GJ, Hakimi P, Hanson RW, Wilson DL (2010) Multi-scale characterization of the PEPCK-C mouse through 3D cryo-imaging. Int J Biomed Imaging. 2010:105984

  30. Ogba N, Doughman YQ, Chaplin LJ, Hu Y, Gargesha M, Watanabe M, Montano MM. (2010) HEXIM1 modulates vascular endothelial growth factor expression and function in breast epithelial cells and mammary gland. Oncogene 29(25):3639-49.

  31. Gangadhariah MH, Mailankot M, Reneker L, Nagaraj RH (2010) Inhibition of methylglyoxal-mediated protein modification in glyoxalase I overexpressing mouse lenses. J Ophthalmol 2010:274317.

  32. Mailankot M, Howell S, Nagaraj RH (2010) Kynurenine inhibits fibroblast growth factor 2-mediated expression of crystallins and MIP26 in lens epithelial cells. Biochim Biophys Acta 1802(7-8):609-20.

  33. Sebastian A, Matsushita T, Kawanami A, Mackem S, Landreth GE, Murakami S. (2011) Genetic inactivation of ERK1 and ERK2 in chondrocytes promotes bone growth and enlarges the spinal canal. J Orthop Res. 29(3):375-9

  34. Alter BJ, Zhao C, Karim F, Landreth GE, Gereau RW 4th. (2010) Genetic targeting of ERK1 suggests a predominant role for ERK2 in murine pain models. J Neurosci. 30(34):11537-47.

  35. Srinivasan R, Zabuawala T, Huang H, Zhang J, Gulati P, Fernandez S, Karlo JC, Landreth GE, Leone G, Ostrowski MC. (2010) Erk1 and Erk2 regulate endothelial cell proliferation and migration during mouse embryonic angiogenesis. PLoS One 4(12):e8283.

  36. Dumitriu B, Bhattaram P, Dy P, Huang Y, Quayum N, Jensen J, Lefebvre V.(2010) Sox6 is necessary for efficient erythropoiesis in adult mice under physiological and anemia-induced stress conditions. PLoS One 5(8):e12088.

  37. Thein DC, Thalhammer JM, Hartwig AC, Crenshaw EB 3rd, Lefebvre V, Wegner M, Sock E (2010) The closely related transcription factors Sox4 and Sox11 function as survival factors during spinal cord development. J Neurochem 115(1):131-41

  38. Ghosal K, Stathopoulos A, Pimplikar SW. (2010) APP intracellular domain impairs adult neurogenesis in transgenic mice by inducing neuroinflammation. PLoS One 5(7):e11866

  39. Liu C, Maejima T, Wyler SC, Casadesus G, Herlitze S and Deneris ES. 2010. Pet-1 is required across different stages of life to regulate serotonergic function. Nature Neurosci 13(10):1190-8

    40. • The Deneris lab developed Pet-1 conditional knockout mice. Using this model, they demonstrated that Pet-1 is required in adult 5-HT neurons.

  40. Dy P, Smits P, Silvester A, Penzo-Mendez A, Dumitriu B, Han Y, de la Motte CA, Kingsley DM, Lefebvre V. 2010 Synovial joint morphogenesis requires the chondrogenic action of Sox5 and Sox6 in growth plate and articular cartilage. Dev Biol. 341(2):346-59

  41. Hawthorne AL, Wylie CJ, Landmesser LT, Deneris ES, Silver J. Serotonergic neurons migrate radially through the neuroepithelium by dynamin-mediated somal translocation. J Neurosci. 2010 30(2):420-30.

  42. Wylie CJ, Hendricks TJ, Zhang B, Wang L, Lu P, Leahy P, Fox S, Maeno H, Deneris ES. Distinct transcriptomes define rostral and caudal serotonin neurons. J Neurosci. 2010 30(2):670-84.

  43. Tapanes-Castillo A, Weaver EJ, Smith RP, Kamei Y, Caspary T, Hamilton-Nelson KL, Slifer SH, Martin ER, Bixby JL, Lemmon VP. A modifier locus on chromosome 5 contributes to L1 cell adhesion molecule X-linked hydrocephalus in mice. Neurogenetics. 2010 11:53-71

  44. Mack JA and Maytin EV. 2010 Persistent Inflammation and Angiogenesis during Wound Healing in K14-Directed Hoxb13 Transgenic Mice. J Invest Dermatol. 130(3):856-65

    Mice from the transgenic facility in publications from previous years